Columns for Reversed-Phase LC Separations in Highly Aqueous Mobile Phases
نویسنده
چکیده
Reversed-phase chromatography is by far the most widely used mode in high performance liquid chromatography (HPLC).1 It allows chromatographers to manipulate the mobile phase by changing organic solvent type, solvent composition and pH; by adding modifiers such as surfactants, chiral reagents, competing bases and ion-pair reagents; or by adjusting experimental conditions such as flow-rate and temperature. Originally, researchers believed that they would need only a few stationary phases to achieve virtually any separation they encountered. Indeed with a C18 column and the ability to adjust the myriad parameters, chromatographers have achieved many successful separations. Still, sometimes analysts can neither find the necessary selectivity nor obtain a rugged and reproducible separation easily, no matter how many parameters they adjust.
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